Abstract

Optical biopsy, such as probe-based endomicroscopy, represents a promising technique that can provide useful intraoperative assessment of cellular imaging instead of conventional physical biopsy and histology. Despite the merits of endomicroscopy, however, it is limited by the high cost of the optical system, difficulties in a flexible approach by a commercial probe, large-area surveillance, and tissue deformation. In this paper, we have developed a low-cost endomicroscopy system with a highly flexible fiber bundle coupled with a distal microlens, mosaicking algorithm, and robotic scanning device for obtaining large-area in vivo cellular imaging to extend the clinical application of endomicroscopy. We have demonstrated that this system can obtain good quality images from ex vivo human stomach tissue. We have also shown the potential of the system to provide a much larger field of view for optical biopsy than conventional endomicroscopy. This could greatly improve the prospects for intraoperative in vivo and in situ evaluation of cellular imaging.

© 2018 Optical Society of America

High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking

Michael Hughes and Guang-Zhong Yang
Biomed. Opt. Express 6(4) 1241-1252 (2015)

Fiber bundle shifting endomicroscopy for high-resolution imaging

Khushi Vyas, Michael Hughes, Bruno Gil Rosa, and Guang-Zhong Yang
Biomed. Opt. Express 9(10) 4649-4664 (2018)

Real-timing processing of fiber bundle endomicroscopy images in Python using PyFibreBundle

Michael R. Hughes
Appl. Opt. 62(34) 9041-9050 (2023)

Fiber-optic large-depth 3D chromatic confocal endomicroscopy

Xuefang Yang, Ye Wang, Hejun Zhang, Haoye Qin, Siyu Wang, Yihang Tong, Keji Zhou, Rui Sun, Shuhua Yue, Xun Chen, Shigang Ding, and Pu Wang
Biomed. Opt. Express 13(1) 300-313 (2022)

Fiber bundle endocytoscopy

Michael Hughes, Tou Pin Chang, and Guang-Zhong Yang
Biomed. Opt. Express 4(12) 2781-2794 (2013)