Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Scanning confocal total internal reflection fluorescence microscopy by using radial polarization in the illumination system

Not Accessible

Your library or personal account may give you access

Abstract

We report on a scanning total internal reflection fluorescence microscope with improved imaging properties. In the illumination system of the developed microscope, radial polarization is employed to obtain the point spread function with a single and circular peak. By using radial polarization, the confocal detection method, which can reduce the background noise of the image, is effectively applicable in the optical system. The observed image of a fluorescent particle with the size of 200nm reveals the improvement of the imaging properties.

© 2009 Optical Society of America

Full Article  |  PDF Article
More Like This
Around-the-objective total internal reflection fluorescence microscopy

Thomas P. Burghardt, Andrew D. Hipp, and Katalin Ajtai
Appl. Opt. 48(32) 6120-6131 (2009)

Confocal total-internal-reflection fluorescence microscopy with a high-aperture parabolic mirror lens

Thomas Ruckstuhl and Stefan Seeger
Appl. Opt. 42(16) 3277-3283 (2003)

Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy

Thomas Ruckstuhl and Stefan Seeger
Opt. Lett. 29(6) 569-571 (2004)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved