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Compensated transillumination

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Abstract

We demonstrate imaging through human tissue in vivo, using a new optical technique, compensated transillumination. Immersion in a scattering medium with absorption and scattering coefficients matched to the tissue is used for drastic improvement of image contrast. The immersion medium is composed of polymer microspheres and methylene blue dye. The optical properties of the medium are matched to those of the tissue by use of a frequency-domain measurement technique. Images of a human hand taken with this technique show the internal structure, including the outlines of bones. The mechanism for the contrast is likely the absence of blood between the bones.

© 1999 Optical Society of America

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