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Three-dimensional phase-contrast imaging by a computed-tomography microscope

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Abstract

We report a technique for measuring the three-dimensional variation of refractive indices in a microscopic sample. The technique is an adaptation of optical computed tomography and is effective in measuring the three-dimensional refractive-index distribution of a nonabsorbing microscopic sample. Our report also includes a discussion of the conditions for the unambiguous application of the technique as well as results of experiments conducted with Aspergillus oryzae (commonly known as green mold) as the sample.

© 1992 Optical Society of America

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