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Two-dimensional phase imaging in the scanning optical microscope

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Abstract

It is shown that an image of absolute object phase may be produced by integrating a differential phase contrast image produced by a large area split detector. Even though integration is being carried out only along the scan lines, a full 2-D phase image is produced by surrounding the object with a medium of uniform path length and resetting the integrator at the beginning of each scan line. Images of a buccal epithelium cell demonstrate the technique.

© 1984 Optical Society of America

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